Characterization of New Molecular Markers of Three Botflies Parasitizing Cervid Hosts

Abstract Specific identification of oestrid larvae is usually problematic not only when using morphobiometric features, but also applying molecular criteria, since very few markers have been described for this group flies. New are needed more reliable species identification, diagnostic purposes, and epidemiological surveys; moreover, they can help in phylogenetic reconstruction. Here, we report the characterization COI, 28S rDNA, ITS1, ITS2 Cephenemyia stimulator from roe deer auribarbis Pharyngomyia picta red deer. The COI rDNA uniform length, while ITSs sequences highly variable at both intraspecific interspecific levels. were longer than those other dipteran by presence simple repeats tandem repeat sequences. In C. ITS1 appeared as two variants, one short long. general, analyzed present low genetic variation high variation. showed greatest amount Phylogenetic analysis demonstrated that characterized differentiate genera Oestridae.